Wheat × Aegilops geniculata interspecific hybrids and amphiploids: Production and fertility assessment

Document Type : Original Article

Authors

1 Ph.D. student of Biotechnology, Isfahan University of Technology, Isfahan, Iran

2 Associate Professor,, Department of Plant Production and Genetics, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

10.34785/J020.2022.514

Abstract

Among the wild relatives of wheat, species in the genus Aegilops have a close relationship to wheat. Aegilops geniculata Roth (2n = 4x = 28; MgMgUgUg) is an allotetraploid, tolerant to various biotic and abiotic stresses that can be introgressed into bread wheat via hybridization and backcrossing. Here, we crossed three bread wheat (Triticum aestivum L., 2n = 6x = 42; AABBDD) cultivars ‘Navid’, ‘Omid’ and ‘Zarin’ with two Ae. geniculata accessions ‘386’ and ‘387’. ‘Zarin’ × Ae. geniculata ‘387’ and ‘Navid’ × Ae. geniculata ‘386’ crosses showed the lowest (11.93%) and highest (48.25%) crossability, respectively. Polyploidy was induced by colchicine treatment in some F1 seedlings and their fertility was compared to non-treated plants. Non-treated hybrid plants with the exception of Zarin’ × Ae. geniculata hybrids also produced F2 seeds. Chromosome configurations in metaphase I of meiosis were mostly univalent along with 3 to 4 rod bivalents. Chromosome numbers in the root tip cells of a sample of F2 seeds ranged from 45 to 65. Fluorescence in situ hybridization (FISH) using (GAA)10 and pTa535-1 as probes, could identify parental chromosomes in the resulting amphiploids.

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