Document Type : Original Article
Authors
1
Associate Professor, Department of Plant Production and Genetics, Faculty of Agriculture, University of Kurdistan, Sanandaj. Iran. Associate Professor, Research Center for Medicinal Plant Breeding and Development, University of Kurdistan, Sanandaj, Iran
2
MSc. graduate. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Zabol University, Zabol, Iran
3
Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Zabol University, Zabol, Iran
4
Associate Professor, Department of Plant Production and Genetics, Faculty of Agriculture, University of Kurdistan, Sanandaj. Iran.
10.34785/J020.2022.007
Abstract
Sahendian savory (Satureja sahendica Bornm) is one of the native species of Iran that contain valuable secondary metabolites. The high value of the active ingredients of Sahendian savory has caused the need for methods for rapid Therefore, in this study, an in vitro tissue culture method was optimized for Sahendian savory. First, explants were prepared from sterile seedlings. Plant growth regulators treatments used for callus induction in MS medium included 2,4-D at three levels with BA (Benzyl Adenine) at four levels or NAA at two levels with BA at five levels (0, 0.5, 1, 2 and 5 mgl-1). BA treatment was also used at five levels for shoot induction. Callus formation of explants showed different reactions under the influence of different hormonal compounds. The explants showed different callogenic reactions under the consequence of different plant growth regulators combinations. The combination of 1 mgl-1 2,4-D plus 1 mgl-1 BA and the combination of 0.5 mgl-1NAA with 0.5 mgl-1 BA showed the highest callus induction rate (100%). The calli produced were green and had a spherical and firm texture. Additionally, the highest number of regenerated shoots (3 shoots) from calli were related to concentrations of 4 and 5 mgl-1 BA. These results can be used as a simple and appropriate technique in the regeneration of Sahendian savory plants for in vitro micropropagation or gene transfer.
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