Effect of different osmotic potential and seed priming levels on some germination characteristics of Paneer phool (Withania coagulans)

Document Type : Original Article

Authors

Assistant Professor, Faculty of Agriculture, Higher Education Complex of Saravan, Saravan, Iran

10.34785/J020.2022.004

Abstract

Paneer phool plant is one of the most important medicinal plants in Balochistan region, which is spread in India, Pakistan and Afghanistan. This plant is used in medicinal and therapeutic cases and is considered as an important cover plant in these areas. The germination of this plant is poor and environmental factors such as drought also have a negative effect on its spread. Therefore, an experiment was conducted with the aim of the effect of osmotic potential and seed priming on some germination characteristics of this plant in a factorial form in a completely randomized design with three replications in 2019. Experimental treatments included four levels of osmotic potential (0, -1, -2 and -3 bar) and eight seed priming treatments including hydropriming, non-priming, priming with gibberellin (200 and 400 ppm), salicylic acid (200 and 400 ppm) and potassium nitrate (200 and 400 ppm). The results showed that seed priming improved root length, shoot length, and germination percentage characteristics. Decreased osmotic potential reduced root length, shoot length and germination percentage. Decreased osmotic potential caused a decrease in seedling length, germination percentage and speed and seed vigor of wind cheese. Priming with gibberellin 200 ppm was the best priming treatment and increased seedling length, germination percentage and speed and seed vigor. In general, priming treatment with gibberellin 200 ppm can be recommended to improve germination traits in this plant.

Keywords

References

Ali, N., Ahmad, B., Bashir, S., Shah, J., Azam, S. and Ahmad, M. 2009. Calcium Channel Blocking Activities of Withania coagulans. African Journal of Pharmacy and Pharmacology, 3: 439-442.
Arshadi, J. and Asgharipour, M.R. 2011. The Effects of Seed Size on Germination and Early Seedling Growth of Pelleted Seeds of Sugar Beet. Applied Sciences Research, 7(8): 1257-1260.
Ashraf, M. and Foolad, M.R. 2005. Pre-sowing seed treatment a shotgun approach to improve germination growth and crop yield under saline and none-saline conditions. Advance Agronomy, 88: 223-271.
Baalbaki, R., Elias, S., Marcos-Filho, J. and Mcdonald, M.B. 2009. Seed Vigor Testing Handbook, AOSA, Ithaca, NY, USA, pp: 341
Bradford, K.J., Steiner, J.J. and Trawatha, S.E. 1990. Seed priming influence on germinating and emergence of pepper seed lots. Crop Science, 30: 718-721.
Burguieres, E., McCu, P., Kwon, Y. and Shetty, K. 2009. Effect of vitamin C and folic acid on seed vigor response and phenolic-linked antioxidation activity. Bioresource Technology, 98: 1393-1404.
Daiya, K. and Kasera, P.K. 2019. Seed germination standardization techniques in Withania coagulans under in-vitro and in-vivo conditions, a critically endangered medicinal plant of Indian Thar desert. Plant Archives, 19(2): 2683-2688.
Darvishi Zeyd Abadi, D., Jalali Javaran, M., Dehghani, H., Baghi Zadeh, A. and Rashidi Monfared, S. 2015. The effect of different combinations of hormonal treatments on seed dormancy failure of different ecotypes of black cumin (Bunium persicum). Seed Science and Research, 2(1): 90-76. (In Persian).
Das, M., Sharma, M. and Kumar, M. 2017. Seed germination of Ashwagandha (Withania somnifera Dunal.): A potential medicinal plant. Medicinal Plants - International Journal of Phytomedicines and Related Industries, 9(2): 102-106.
Dermi Kaya, M., Okçu G., Atak, M., Çikili, Y. and Kolsarici, O. 2006. Seed treatment to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24: 291-295.
Dhoran, V.S. and Gudadhe, S.P. 2012. Effect of plant growth regulators on seed germination and seedling vigour in Asparagus sprengeri Regelin. Biological Sciences, 1(7): 6-10.
Duman, I. 2006. Effect of seed priming with PEG and K3PO4 on germination and seedling growth in Lettuce. Pakistan Journal of Biology Science, 9(5): 923-928.
Farooq, M., Wahid, A. and Lee, D.J. 2009. Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiologiae Plantarum, 31(5): 937-945.
Farooq, M.S.M.A., Basra, S.M.A. and Ahmad, N. 2007. Improving the performance of transplanted rice by seed priming. Plant Growth Regulation, 51(2): 129-137.
Gupta, P.C. 2012. Withania coagulans (Stocks), Residual effects of farm yard manure on soil properties in spring season, Chitwan. Nepal Agricultural Reaserch Councli, 1: 165-171.
Harris, D. 2006. Development and testing of on-farm seed priming. Advances in Agronomy, 90: 129-178.
Hemalatha, S., Kumar, R. and Kumar, M. 2008. Withania coagulans Dunal: A Review.Pharmacognosy Review, 2: 351-358.
ISTA (International Seed Testing Association). 2009. International Role for Seed Testing. International Seed Testing Association. Bassersdorf, Switzerland.
Kafi, M., Borzuie, A., Salehi, M., Kamandi, M., Masumi, A. and Nabati, J. 2018. Physiology of Environmental Stresses in Plants. Jihad Daneshgahi Publications of Mashhad. 502 p. (In Persian).
Kaur, S., Gupta, A.K. and Kaur, N. 2002. Effect of osmo- and hydropriming of chickpea seeds on the performance of crop in the field. International Chickpea and Pigeonpea Newsletter, 9: 15-17.
Koocheki, A.R. and Sarmad Nia, G. 2013. Crop physiology. Ferdowsi University of Mashhad Publications. (In Persian).
Michel, B.E. and Kaufmann, M.R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51: 914-916. 
Patade, V.Y., Bhargava, S. and Suqruasanna, P. 2009. Halopriming imparts tolerance to salt and PEG induced drought stress in sugarcane. Agriculture, Ecosystem and Environment, 134: 24-28.
Pill, W.G., Collins, C.M., Goldberger, B. and Gregory, N. 2009. Responses of non-primed or primed seeds of ‘Marketmore 76’cucumber (Cucumis sativus L.) slurry coated with Trichoderma species to planting in growth media infested with Pythium aphanidermatum. Scientia Horticulture, 121(1): 54-62.
Saxena, N.P. 2003. Management of agricultural drought: agronomic and genetic options. Science Publishers, Inc.
Sharma, R.K., Sharma, S. and Sharma, S.S. 2006. Seed germination behavior of some medicinal plants of Lahaul and Spiti cold desert (Himachal Pradesh): implications for conservation and cultivation. Current Sciences, 90(8): 1113-1118.
Plant Production and Genetics
Volume 3, Issue 2 - Serial Number 4
September 2022
Pages 235-246

Files

Share

How to cite

Statistics