Molecular and biochemical evaluation of some traits in rapeseed cultivars under drought stress condition

Document Type : Original Article

Authors

1 Professor. Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

2 Ph.D. Graduate.Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

3 MSc. graduate. Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

Abstract

Oilseeds are the second largest food reserves in the world after cereals. Rapeseed cultivars were drought sensitive during germination and during pod growth stages and the greatest increase in damage occurs simultaneously with the lack of water during the growth of newly established young seedlings. Seeds of 6 rapeseed cultivars (including: Kodiak, Jerry, Brutus, Jacomo, Julius, Traviata) were tested in the research greenhouse of Gorgan University of Agricultural Sciences and Natural Resources as factorial experiments with a completely randomized design with 4 replications. Drought treatment levels including 6-, 4-, 2-, 1-, 0.5-bar were irrigated according to the equivalent amount of water required in the repetitions of each treatment. Sampling was performed to calculate yield index and related traits, oil percentage, TBARM biochemical index, chlorophyll and proline content as well as random sampling to perform molecular studies and study of gene expression. The results showed that with increasing stress levels in rapeseed cultivars, grain yield and related traits, oil content and chlorophyll content decreased. It was also observed that with increasing stress, TBARM and proline index were produced to a greater extent. In the case of ACCase and SAD genes, the highest gene expression was related to Traviata cultivar at 4-bar stress level and also the results obtained from P5CS1 and PDH genes showed that with increasing drought stress in rapeseed cultivars, gene expression was also increased. There was an increase in them.

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