Document Type : Original Article
Authors
1
Associate Professor, Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2
Ph.D. Student,, Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
3
MSc. graduate. , Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Abstract
Wheat is one of the most important crops, accounting for about 16% of arable land. One of the most important diseases that cause damage to wheat is Septoria tritici. The disease is a serious and ongoing challenge to wheat production in temperate regions around the world. The most basic strategy to control this disease is to find resistant genotypes and develop cultivation of them. In recent years, studies have evaluated the role and expression of some genes involved in the disease resistance process and have clarified the strengths and weaknesses of the plant and the pathogen in the path of the disease. In the present study, to confirm the differential expression of some isolated genes (from the cDNA-AFLP project), the expression pattern of Acetolactate synthase, calnexin, and GDP-mannose 3 and 5 epimerase genes were evaluated by quantitative real-time polymerase chain reaction. After infection of a resistant line (No. 10) and a susceptible wheat cultivar (Tajan) with the fungus, expression of the studied genes was measured at 9-time points (before inoculation as control, six and 12 hours, one, two, three, four, five and seven days after inoculation). The results, while confirming the results of cDNA-AFLP, showed that all three genes play an important role in inducing the resistance reaction to Septoria disease through the production of ascorbic acid as an antioxidant in removing ROS, maintaining amino acid homeostasis and controlling correct folding of proteins are responsible.
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