Identification of the key CPT1 gene in natural rubber biosynthetic pathway in Chodrilla juncea and extraction of rubber by enzymatic method

Document Type : Original Article

Authors

1 Ph.D. student, Department of Plant Production and Genetics, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran

2 Associate Professor, Department of Plant Production and Genetics, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran

10.22034/plant.2024.141894.1119

Abstract

Natural rubber is a vital material for the industry, which is usually obtained from the rubber tree (Hevea brasiliensis). Considering that it is not possible to grow this tree in many areas, it seems necessary to find an alternative source for this valuable material. In this study, the presence of natural rubber in the Ghandaroun plant with the scientific name Chondrilla juncea was investigated from a molecular and biochemical point of view. The results showed that a significant amount of natural rubber can be extracted from the roots of this plant in the autumn season by using the enzyme method. Also, in the present study, the key gene of cis-prenyl transferase (CPT) involved in the biosynthesis of natural rubber was identified and sequenced using different primers on the genomic DNA of this plant. After assembling the sequences obtained, the complete sequence of this gene was obtained. Comparing the nucleotide and protein sequences of this gene showed a great similarity to the CPT gene in other plants of this family and even the rubber tree. This article represents the first report of natural rubber production in Ghandaroun plant. Further investigations and breeding programs, it may position this plant as a novel commercial source for natural rubber production.

Keywords

Plant Production and Genetics
Volume 5, Issue 2 - Serial Number 8
November 2024
Pages 287-298

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