Determination of ploidy level of plantlets obtained from Cyclamen persicum anther culture

Articles in Press

Document Type : Original Article

Authors

1 MSc. graduateŁˆ Department of Plant Production and Genetics, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Associate Professor, Department of Plant Production and Genetics, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

10.22034/plant.2025.142160.1128

Abstract

The cyclamen plant (Cyclamen persicum L.) is a perennial plant with winter flowering. In this study, 14 plantlets obtained from cyclamen anther culture were investigated using various methods, including determination of the number of the chloroplasts in the stomatal guard cells, stomatal guard cell density and size, flow cytometry, and molecular markers No significant differences were observed in the number of chloroplasts, stomatal guard cell density, or the length and width of stomatal guard cells between the haploid candidate plantlets and the mother plantlets. The results of flow cytometry indicated that the ploidy level of the mother plant and all plants obtained from anther culture was the same. Additinonally, this study evaluated the origin and genetic diversity of plantlets obtained from cyclamen anther culture using 5 ISSR primers. Analysis of the molecular data revealed that none of the samples obtained from cyclamen anther culture were genetically identical to the maternal plant. Differences were observed between the samples and the maternal plant at least in some marker loci. As a result, it was concluded that none of the plantlets were derived from the anther wall and all of them originated from pollen grains. However, the results of chloroplast counting in the stomatal guard cells, stomatal guard cell density and size on the lower leaf surface, and flow cytometry confirmed the diploid nature of the plants obtained from anther culture., This suggests that all the plantlets obtained from the anther culture of cyclamen are spontaneous diploids.

Keywords

Plant Production and Genetics

Articles in Press, Accepted Manuscript
Available Online from 06 January 2025

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