شناسایی ژن کلیدی CPT1 در مسیر بیوسنتزی لاستیک طبیعی در گیاه Chodrilla juncea و استخراج لاستیک به روش آنزیمی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، گروه تولید و ژنتیک گیاهی ، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

2 دانشیار، گروه تولید و ژنتیک گیاهی ، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران

10.22034/plant.2024.141894.1119

چکیده

لاستیک طبیعی یک ماده حیاتی برای صنعت است که به‌طور معمول از درخت کائوچو (Hevea brasiliensis) به‌ دست می-آید. با توجه این‌که امکان کشت این درخت در بسیاری از مناطق وجود ندارد، یافتن منبع جایگزین برای این ماده باارزش ضروری به نظر می‌رسد. در این مطالعه وجود لاستیک طبیعی در گیاه قندرون با نام علمی Chondrilla juncea از لحاظ مولکولی و بیوشیمیایی بررسی شد . نتایج نشان داد با استفاده از روش آنزیمی می‌توان مقدار قابل‌ملاحظه‌ای لاستیک طبیعی در فصل پاییز از ریشه‌ی این گیاه استخراج نمود. همچنین در مطالعه حاضر ژن کلیدی سیس‌پرنیل ترانسفراز (CPT) دخیل در بیوسنتز لاستیک ‌طبیعی با استفاده از آغازگرهای مختلف روی DNA ژنومی این گیاه با روش گام‌زنی شناسایی و توالی یابی شد. پس از سرهم‌بندی قطعات به‌دست‌آمده، توالی کامل این ژن به دست آمد. مقایسه توالی نوکلئوتیدی و پروتئینی این ژن شباهت زیادی به ژن CPT در سایر گیاهان این خانواده و حتی درخت کائوچو نشان داد. نتایج این تحقیق اولین گزارش تولید لاستیک طبیعی در گیاه قندرون است. با مطالعات بیشتر در این زمینه و برنامه‌های به‌نژادی شاید بتوان این گیاه را به‌عنوان منبع جدید تجاری برای تولید لاستیک طبیعی معرفی نمود.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of the key CPT1 gene in natural rubber biosynthetic pathway in Chodrilla juncea and extraction of rubber by enzymatic method

نویسندگان [English]

  • Benyamin Jalili 1
  • Asghar Mirzaie-Asl 2
  • Hedayat Bagheri 2
1 Ph.D. student, Department of Plant Production and Genetics, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran
2 Associate Professor, Department of Plant Production and Genetics, Faculty of Agriculture, University of Bu-Ali Sina, Hamedan, Iran
چکیده [English]

Natural rubber is a vital material for the industry, which is usually obtained from the rubber tree (Hevea brasiliensis). Considering that it is not possible to grow this tree in many areas, it seems necessary to find an alternative source for this valuable material. In this study, the presence of natural rubber in the Ghandaroun plant with the scientific name Chondrilla juncea was investigated from a molecular and biochemical point of view. The results showed that a significant amount of natural rubber can be extracted from the roots of this plant in the autumn season by using the enzyme method. Also, in the present study, the key gene of cis-prenyl transferase (CPT) involved in the biosynthesis of natural rubber was identified and sequenced using different primers on the genomic DNA of this plant. After assembling the sequences obtained, the complete sequence of this gene was obtained. Comparing the nucleotide and protein sequences of this gene showed a great similarity to the CPT gene in other plants of this family and even the rubber tree. This article represents the first report of natural rubber production in Ghandaroun plant. Further investigations and breeding programs, it may position this plant as a novel commercial source for natural rubber production.

کلیدواژه‌ها [English]

  • : Cis-prenyl transferase
  • ghandaroun
  • rubber tree
  • sequencing

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