بهینه‌سازی عملکرد و صفات دفاعی نعناع گریپ‌فروتی (Mentha suaveolens×piperita) تحت شرایط تنش خشکی با کاربرد قارچ میکوریزا و نانوذرات کربن کوانتوم دات

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانش آموخته دکتری، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران

2 استاد، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران

3 دانشیار، گروه مهندسی علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران

4 دانشجوی کارشناسی ارشد، گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران

10.22034/plant.2024.142333.1130

چکیده

پژوهشی با هدف بررسی اثر نانوذرات کربن کوانتوم دات و قارچ میکوریزا آربوسکولار بر رشد و پاسخ‌های بیوشیمیایی نعناع گریپ‌فروتی تحت تنش خشکی انجام شد. آزمایش‌ به ‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه سطح تنش خشکی (90، 70 و 50 درصد ظرفیت زراعی) و شش تیمار کودی شامل بدون کود (شاهد)، قارچ میکوریزا آربوسکولار (AMF)، کربن کوانتوم‌دات 5 پی‌پی‌ام (CQD5)، کربن‌کوانتوم دات 10 پی‌پی‌ام (CQD10)، AMF+CQD5، و AMF+CQD10 در 4 تکرار اجرا شد. نتایج نشان داد که ترکیب AMF+CQD10 در شرایط آبیاری نرمال، به‌طور قابل توجهی منجر به بهبود رشد گیاه و افزایش عملکرد ماده خشک نعناع گریپ‌فروتی گردید. با این حال، در شرایط تنش شدید خشکی، گیاهان شاهد با بالاترین سطح آسیب اکسیداتیو (محتوای بالای مالون‌دی‌آلدهید و پراکسید هیدروژن) مواجه بودند، در حالی‌که تیمارهای حاویAMF + CQDباعث کاهش این نشانگرهای استرس شدند. علاوه بر این، در شرایط تنش خشکی، کاربرد AMF+CQD10 منجر به افزایش محتوای فنل، فلاونوئید، پرولین، کربوهیدرات‌های محلول، آنتوسیانین و اسید اسکوربیک شد که نشان‌دهنده افزایش تحمل به خشکی در نعناع گریپ‌فروتی بود. علاوه بر این، در شرایط تنش با کاربرد AMF+CQD10، افزایش محتوای فنل، فلانوئید، پرولین، کربوهیدرات‌های محلول، آنتوسیانین و اسید آسکوربیک نشانگر بهبود مقاومت گیاه در برابر خشکی است. بطور خلاصه، کاربرد ترکیب قارچ میکوریزا آربوسکولار و غلظت 10 پی‌پی‌ام نانوذرات کربن کوانتوم دات می‌تواند به‌طور موثری صفات دفاعی و بیوشیمیایی نعناع گریپ‌فروتی را در مواجهه با تنش خشکی بهبود بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

Optimizing the performance and defensive traits of grapefruit mint (Mentha suaveolens × piperita) under drought stress conditions using arbuscular mycorrhizal fungi and carbon quantum dots

نویسندگان [English]

  • mohammad haghaninia 1
  • Abdollah Javanmard 2
  • Farzad Rasouli 3
  • Fatemeh Hazrati-Haselghoeiafshar 4
1 Ph.D. graduate, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, , Maragheh, Iran
2 Professor, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, , Maragheh, Iran
3 Associate Professor, Department of Horticultural Science, Faculty of Agriculture, University of Maragheh, Maragheh, Iran
4 MSc. student, Department of Plant Production and Genetics, Faculty of Agriculture, University of Maragheh, , Maragheh, Iran
چکیده [English]

With the growing challenges caused by environmental stresses such as drought, improving strategies to mitigate these stresses has become increasingly critical. This study aimed to evaluate the effects of carbon quantum dots (CQDs) and arbuscular mycorrhizal fungi (AMF) on the growth and biochemical responses of grapefruit mint under drought stress. A factorial experiment was conducted in a completely randomized design with three levels of drought stress (90%, 70%, and 50% of field capacity) and six fertilizer treatments, including no fertilizer (control), AMF, CQD at 5 ppm (CQD5), CQD at 10 ppm (CQD10), AMF + CQD5, and AMF + CQD10, in four replications. Results showed that the combined application of AMF + CQD10 significantly improved plant growth and increased the dry matter yield of grapefruit mint under optimal irrigation conditions. Under severe drought stress, control plants exhibited the highest levels of oxidative damage, as indicated by elevated malondialdehyde and hydrogen peroxide content. However, AMF + CQD treatments effectively reduced these stress markers. Additionally, under drought stress conditions, the application of AMF + CQD10 led to significant increases in phenol, flavonoid, proline, soluble carbohydrates, anthocyanin, and ascorbic acid contents, suggesting enhanced drought tolerance in grapefruit mint. Also, the activity of antioxidant enzymes such as ascorbate peroxidase, superoxide dismutase and catalase were also strengthened in this treatment. In conclusion, the combined application of AMF and CQD10 can effectively improve defense and biochemical characteristics of grapefruit mint under drought stress.

کلیدواژه‌ها [English]

  • Abiotic stress
  • Dry matter yield
  • Sustainable agriculture
  • Secondary metabolites
  • Nanoparticles

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