بررسی واکنش افتراقی برخی ژن‌ها تحت‌تاثیر تنش اکسیداتیو در سه رقم گندم نان

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه اصلاح نباتات و بیوتکنولوژی ، دانشکده تولید گیاهی، دانشگاه منابع طبیعی و علوم کشاورزی گرگان، گرگان، ایران

2 استاد، گروه اصلاح نباتات و بیوتکنولوژی ، دانشکده تولید گیاهی، دانشگاه منابع طبیعی و علوم کشاورزی گرگان، گرگان، ایران

3 دانش آموخته دکتری، گروه اصلاح نباتات و بیوتکنولوژی ، دانشکده تولید گیاهی، دانشگاه منابع طبیعی و علوم کشاورزی گرگان، گرگان، ایران

4 دانشجوی دکتری، گروه اصلاح نباتات و بیوتکنولوژی ، دانشکده تولید گیاهی، دانشگاه منابع طبیعی و علوم کشاورزی گرگان، گرگان، ایران

10.22034/plant.2025.143480.1156

چکیده

با هدف ارزیابی تاثیر سطوح تنش اکسیداتیو بر برخی ژن‌ها مطالعه‌ای بر روی سه رقم گیاه گندم (احسان، کلاته و گنبد) در گلخانه تحقیقاتی دانشگاه علوم کشاورزی و منابع طبیعی گرگان صورت گرفت. در این راستا، دو آزمایش مجزا مشتمل بر بررسی سطوح نیترات نقره (در سه سطح 0، 1 و 2 میلی‌مولار) بعنوان عامل اکسیداتیو و اسید آسکوربیک (در سه سطح 0، 10 و 20 میلی‌مولار) بعنوان پاداکسیدان عمومی انجام گرفت. آزمایش دوم برای تعیین اثر اختصاصی یون سوپراکسید و اکسیژن اتمی بعنوان عوامل سیگنالی یا اکسیداتیو انجام شد که از پاک‌کننده‌های اختصاصی آن‌ها به ترتیب تیرون (با غلظت 0، 2 و 4 میلی مولار) و دبکو (با غلظت 0، 15 و 30 میلی مولار) بعنوان پیش تیمار نیترات نقره استفاده گردید. اعمال تمامی تیمارها با انجام محلول پاشی (مه پاش) در محدوده مرحله 60 زادکس (8 برگی) انجام و به منظور افزایش دقت از طرح کامل تصادفی استفاده شد. کلیه تیمارهای آزمایشی با هدف ارزیابی بیان برخی ژن‌ها اعم از CAT، GPX، MAPK3 و MAPK6 انجام گرفت. نتایج نشان داد. بیان نسبی ژن‌های MAPK3 و MAPK6 همبستگی مثبتی با افزایش میزان آسکوربیک اسید در رقم احسان نشان داد. همچنین مشخص شد MAPK6 از طریق مسیرهای سیگنالینگ خود باعث القای بیان ژن و افزایش بیان کاتالاز می‌گردد. در عین حال مشخص شد رقم احسان نسبت به سایر ارقام، تحت‌تاثیر آسکوربیک اسید عملکرد بهتری نسبت به تاثیر پاداکسیدان‌های اختصاصی داشته و در شرایط تنش واکنش بهتری دارد.

کلیدواژه‌ها

عنوان مقاله [English]

Study of the differential response of certain genes to oxidative stress in three bread wheat cultivars

نویسندگان [English]

  • Somaye Damavandi 1
  • Saied Navabpour 2
  • Abolfazl Mazandarani 3
  • Mojtaba Molaee 4
1 MSc. student,, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Natural Resources and Agricultural Sciences, Gorgan, Iran
2 Professor,, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Natural Resources and Agricultural Sciences, Gorgan, Iran
3 Ph.D. graduate,, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Natural Resources and Agricultural Sciences, Gorgan, Iran
4 Ph.D. student,, Department of Plant Breeding and Biotechnology, Faculty of Plant Production, Gorgan University of Natural Resources and Agricultural Sciences, Gorgan, Iran
چکیده [English]

A study was conducted on three wheat cultivars (Ehsan, Kalateh, and Gonbad) in the research greenhouse of Gorgan University of Agricultural Sciences and Natural Resources to evaluate the impact of oxidative stress levels on specific genes. Two separate experiments were carried out. The first experiment involved studying silver nitrate levels (at 0, 1, and 2 mM) as an oxidative agent and ascorbic acid (at 0, 10, and 20 mM) as a general antioxidant. The second experiment aimed to determine the specific effects of superoxide ion and atomic oxygen as signaling or oxidative agents. Specific scavengers, Tirone (at 0, 2, and 4 mM) and Debco (at 0, 15, and 30 mM), were used as pretreatment of silver nitrate. All treatments were applied through foliar spraying at the 60 Zadex stage (8 leaves) using a completely randomized design for accuracy. The study evaluated the expression of genes including CAT, GPX, MAPK3, and MAPK6. Results indicated a positive correlation between the relative expression of MAPK3 and MAPK6 genes and increased levels of ascorbic acid in the Ehsan cultivar. It was observed that MAPK6 induced gene expression and enhanced catalase expression through its signaling pathways. Additionally, the Ehsan cultivar exhibited superior performance under the influence of ascorbic acid compared to specific antioxidants and showed better responses to stress conditions than the other cultivars.

کلیدواژه‌ها [English]

  • Catalase
  • Glutathione peroxidase
  • Oxidative stress
  • Silver nitrate
  • Wheat

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