اثر کاربرد باکتری های محرک رشد بر عملکرد و اجزای عملکرد گندم تحت شرایط تنش خشکی

نوع مقاله : مقاله پژوهشی

نویسنده

دانش آموخته کارشناسی ارشد، داشکده کشاورزی و منابع طبیعی دانشگاه خلیج فارس، بوشهر، ایران

چکیده

این پژوهش به‌منظور بررسی تأثیر کاربرد باکتری­های محرک رشد بر عملکرد و اجزای عملکرد گندم، رقم الموت، تحت شرایط تنش خشکی در سال زراعی 97-1396 در مزرعه تحقیقاتی دانشگاه رازی کرمانشاه اجرا شد. این پژوهش به‌صورت فاکتوریل در چارچوب طرح بلوک­های کامل تصادفی با چهار تکرار انجام شد. عامل نخست آبیاری در سه سطح (بر پایه 80، 120 و 160 میلی­متر تبخیر از تشتک تبخیر کلاس A) و عامل دوم باکتری­های محرک رشد در چهار سطح (PGU19, PGU18, PGU17, PGU16) به همراه یک تیمار شاهد (بدون باکتری) بودند. تعداد 20 جدایه از دید ویژگی­های برجسته محرک رشدی و تحمل به خشکی در شرایط آزمایشگاه موردبررسی قرار گرفتند و از میان آ­ن­ها چهار جدایه که قابلیت ویژگی‌های محرک رشد و تحمل به خشکی بالاتری داشتند برای تلقیح انتخاب شدند. بذر­ها در زمان کاشت با باکتری­های سودوموناس فلورسنت (غلظت 108 سلول در هر گرم باکتری) تلقیح شدند. برآیندهای تجزیه واریانس نشان داد که اثرات اصلی همه فاکتورها بر صفات موردمطالعه معنی­دار بود و اثرات متقابل آبیاری و باکتری بر صفات ارتفاع بوته، تعداد سنبله و تعداد دانه در سنبله معنی­دار بود. با افزایش تنش خشکی عملکرد و اجزای عملکرد کاهش یافت. در همه سطوح تنش، جدایه­های مختلف باکتری موجب افزایش همه صفات موردبررسی در سنجش با شاهد شدند ولی از تیمار 80 میلی‌متر تبخیر از کلاس A به بعد کاربرد باکتری اثر معنی­داری بر صفات مذکور نداشت.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of plant growth promoting rhizobacteria (PGPRs) applying on yield and yield components of Almute wheat under drought stress condition

نویسنده [English]

  • Dariush Safari
Graduate Student, Faculty of Agriculture and Natural Resources, University of the Persian Gulf, Bushehr, Iran
چکیده [English]

This study was carried out to investigate the effect of application of growth stimulating bacteria on yield and yield components of wheat, Alamut cultivar under drought stress conditions in a research farm of Razi University of Kermanshah in the period of 2018-2017. Experimental design of this research was factorial based on randomized complete blocks design with four replications. First factor included of Irrigation at three levels (based on 80, 120 and 160 mm evaporation from class A evaporation pan) and second factor included of growth promoting bacteria in four levels (PGU19, PGU18, PGU17, PGU16) with a control (no bacteria). 20 isolates were studied in terms of the important characteristics of plant growth promoter and drought tolerance in laboratory conditions. Four strains which were superior to others based on growth-promoting properties and drought tolerance were selected. At planting time, seeds were inoculated with suspension of selected Pseudomonas strains (108 CFU/ml). The results of variance analysis showed that the main effects of all factors on the studied traits were significant and the interaction effects of bacteria and irrigation were significant on plant height, number of spikes and number of seeds per spike. With increasing drought stress, yield and yield components decreased. Different bacterial isolates increased all traits compared to control at all levels of stress, but after 80 mm evaporation from class A, bacterial application had no significant effect on mentioned traits.

کلیدواژه‌ها [English]

  • Fluorescent Pseudomonads
  • Moisture Stress
  • Alamute
  • Yield

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